【文献标题】 Tumor-associated macrophages promote cancer stem cell-like 4 properties via transforming growth factor-beta1-induced 5 epithelial–mesenchymal transition in hepatocellular carcinoma

【作者】 Qing-Min Fan，Ying-Ying Jing，Guo-Feng Yu，et.al

【作者单位】 第二军医大学（The Second Military Medical University）

【文献中引用产品】

小鼠转化生长因子β1（TGF-β1） ELISA试剂盒

【关键词】 Hepatocellular carcinoma，Tumor-associated macrophages，Cancer stem cells，Epithelial to mesenchymal transition，Transforming growth factor-beta1

【DOI】 dx.doi.org/10.1016/j.canlet.2014.05.008

【影响因子(IF)】 6.50

【 出版期刊】 《Cancer Letters》

【产品原文引用】

20 ng/ml IL-4 and 100 ng/ml LPS plus 20 ng/ml IFN-c was used to stimulate two types of macrophages for 24 h, respectively. Then the supernatants of RAW264.7 cells were centrifuged and collected. Amounts of TGF-b1  secreted by macrophage was measured using commercial ELISA kits ( Shanghai Hengyuan Biotechnology Co., Ltd. ), according to the manufacturer’s instructions.

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