【文献标题】 Hepatic Stellate Cells Secreted Hepatocyte Growth Factor Contributes to the Chemoresistance of Hepatocellular Carcinoma

【作者】 Guofeng Yu，Yingying Jing，Xingrui Kou，et.al

【作者单位】 第二军医大学（The Second Military Medical University）

【文献中引用产品】

人肝细胞生长因子（HGF）ELISA试剂盒

人转化生长因子β（TGF- β）ELISA试剂盒

【DOI】 doi:10.1371/journal.pone.0073312

【影响因子(IF)】 3.02

【 出版期刊】 《PLOS ONE》

【产品原文引用】

Conditioned medium and ELISA

Cultured media (CM) were collected from LX-2 cells cultured in 2D culture dishes and centrifuged at 1000g for 5 minutes to obtain the supernatant. LX-2 CM was incubated with HGF antibody (GTX10678, 0.5 µg/ml) for 24h at 4oC to obtain HGFdepleted LX-2 CM. The level of HGF and TGF-β in CM waere determined by using human HGF ELISA kit (HY10158E,Shanghai HengYuan Biological Technology Co., Ltd) and TGF-β ELISA kit  (HY10108E, Shanghai HengYuan Biological Technology Co., Ltd) according to manufacturer’s instructions.

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