【文献标题】 Expression of Tumor-Related Macrophages and Cytokines After Surgery of Triple-Negative Breast Cancer Patients and its Implications

【作者】 Jianguo Wang，Hongwu Chen，Xingwu Chen，et.al

【作者单位】 湘潭市中心医院（The Central Hospital of Xiangtan City）

【文献中引用产品】

人C-C基序趋化因子5(CCL5)ELISI试剂盒

人巨噬细胞炎性蛋白2（MIP-2）ELISA试剂盒

【关键词】Adrenal Gland Neoplasms，Macrophages，Occlusal Adjustment

【DOI】 DOI: 10.12659/MSM.895386

【影响因子(IF)】 1.99

【 出版期刊】 《MEDICAL SCIENCE MONITOR》

【产品原文引用】

Enzyme-linked immunosorbent assay (ELISA)

Test kits for human IL-6, IL-10, Il-12 and IL-1b were purchased from Ruiboao Biotech, Guangzhou, China. Test kits for human CCL-5  and MIP-2  were products of Hengyuan Biotech, Shanghai, China . ELISA was performed according to the manual instructions. In brief, total proteins were extracted from both CD68 high infiltration and low infiltration groups. Both standard samples and tissue samples were added into 96-well plates, followed by the addition of 0.1 mL biotin-labelled antibody and 60-min incubation at 37℃. After gentle washing in 0.01 M TBS, ABC reagents were added into each well for further 30-min ncubation. The color was developed in the dark by TMB reagents. After stopping, a microplate reader quantified the optical density value at 470 nm for further analysis.

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