【文献标题】Unraveling the mechanism of interaction: accelerated phenanthrene degradation and rhizosphere bioflm/iron plaque formation infuenced by phenolic root exudates

【作者】Dan A、 Yifei Zhang、Hanjie Huang et.al

【作者单位】仲恺大学

【文献中引用产品】

双加氧酶（DIO）ELISA试剂盒

邻苯二酚1，2-双加氧酶（C12O）ELISA试剂盒

【关键词】 Phenanthrene，Phenolic root exudates，Bioflm，Iron plaque，Co-metabolic degradation，Rhizosphere microenvironment

【DOI】doi.org/10.1007/s11356-024-33349-z

【影响因子(IF)】0

【出版期刊】《Environmental Science and Pollution Research (2024）》

【产品原文引用】

  Chemical oxygen demand (COD) and ammonia nitrogen (NH4+-N) concentrations were measured using a water quality analyzer (YCNPN-4H, Shanghai Jinzhuang Instrument Co., LTD., China). The activities of PHE-degrading enzymes, including dioxygenase (DIO), 1-hydroxy-2-naphthalene formaldehyde (salt) hydroxylase (1H2N), and C12O, were assayed using specifc ELISA kits (Shanghai Huding Biotechnology Co., LTD., China), according to the manufacturer’s instructions. The corresponding enzyme activities were determined using a microplate absorbance reader (iMark, Bio-Rad, USA) at 450 nm.

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