闍ｱ譁圽錐遘ｰphospho-PPAR alpha (Ser12)
荳ｭ譁圽錐遘ｰ逎ｷ驟ｸ蛹ɩalpha;蝙圽霑ɩｰｧ蛹夜ɩ豢ｻ蛹門｢樒函蜿嶺ｽ捺蒲菴直br />蛻ｫ    蜷恒PAR alpha (phospho-Ser12); PPAR alpha (phospho-S12); p-PPAR alpha (Ser12); p-PPAR alpha (S12); hPPAR; MGC2237; MGC2452; NR1C1; Nuclear receptor subfamily 1 group C member 1; Peroxisome Proliferator Activated Receptor alpha; PPAR; PPAR alpha; PPARA; OTTHUMP00000197740; OTTHUMP00000197741; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; PPARA_HUMAN; PPARalpha.
隸ｴ 譏荵ｦ100ul
莠ｧ蜩∫ｱｻ蝙狗｣ｷ驟ｸ蛹匁蒲菴ɩnbsp;
遐皮ｩｶ鬚圽沺閧ｿ逖､  扈ɩ逕溽黄  蜈咲稔蟄ｦ  霓ｬ蠖戊ｰ鰍蝗蟄ɩnbsp; 豼€驟ｶ蜥檎｣ｷ驟ｸ驟ｶ
謚嶺ｽ捺擂貅審abbit
蜈矩嚀邀ｻ蝙輝olyclonal
莠､蜿牙渚蠎禰uman, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig,
莠ｧ蜩∝ｺ皮畑WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 ⣨育浹陷｡蛻・援髴€蛛壽蒲蜴滉ｿｮ螟搾ｼɩnbsp;
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
蛻蟄駱驥圽2kDa
扈ɩ螳壻ｽ咲ｻɩ譬ｸ
諤ｧ    迥ｶLyophilized or Liquid
豬ɩnbsp;   蠎ｦ1mg/ml
蜈逍ｫ 蜴檪LH conjugated Synthesised phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12:PL(p-S)PL
莠ɩnbsp;   蝙紀gG
郤ｯ蛹匁婿豕病ffinity purified by Protein A
蛯ｨ 蟄ﴚ豸ｲ0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
菫晏ｭ俶擅莉ｶStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
莠ｧ蜩∽ｻ狗ｻ甲ackground:
Peroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.

Function:
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.

Subunit:
Heterodimer; with RXRA. This heterodimerization is required for DNA binding and transactivation activity. Interacts with AKAP13, LPIN1 and PRDM16. Also interacts with PPARBP coactivator in vitro. Interacts with CITED2; the interaction stimulates its transcriptional activity (By similarity). Interacts with NCOA3 and NCOA6 coactivators. Interacts with ASXL1 AND ASXL2.

Subcellular Location:
Nucleus.

Tissue Specificity:
Skeletal muscle, liver, heart and kidney.

Similarity:
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
Q07869

Gene ID:
5465

Database links:
Entrez Gene: 5465 Human

Entrez Gene: 19013 Mouse

Entrez Gene: 25747 Rat

Omim: 170998 Human

SwissProt: Q07869 Human

SwissProt: Q6I9S0 Human

SwissProt: P23204 Mouse

SwissProt: Q542P9 Mouse

SwissProt: P37230 Rat

Unigene: 103110 Human

Unigene: 710044 Human

Unigene: 212789 Mouse

Unigene: 9753 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or di

莠ｧ蜩∝崟迚/td>

Sample: Liver (Rat) Lysate at 40 ug Primary: Anti-phospho-PPAR alpha (Ser12) (bs-4055R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD Sample: Heart (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti-phospho-PPAR alpha (Ser12) (bs-4055R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD Sample: Colon carcinoma (Human) Lysate at 40 ug Embryo (Mouse) Lysate at 40 ug Primary: Anti- phospho-PPAR alpha (Ser12) (bs-4055R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 52 kD Observed band size: 52 kD Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37邃for 20 min; Incubation: Anti-phospho-PPAR alpha(Ser12) Polyclonal Antibody, Unconjugated(bs-4055R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining phosphopeptide non phosphopeptide