技术文章
FluorPen FP110手持式叶绿素荧光仪相关文献
阅读:2451 发布时间:2019-9-19FluorPen FP110手持式叶绿素荧光仪用于实验室、温室和野外快速测量植物叶绿素荧光参数,具有便携性强、度高、性价比高等特点;双键操作,具图形显示屏,内置锂电和数据存储,广泛应用于研究植物的光合作用、胁迫监测、除草剂检测或突变体筛选,还可用于生态毒理的生物检测,如通过不同植物对土壤或水质污染的叶绿素荧光响应,找出敏感植物作为生物传感器用于生物检测。FP110配备多种叶夹型号,用于不同的样品与研究
● 参考文献
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附: OJIP 参数及计算公式
Bckg = background
Fo = F50μs; fluorescence intensity at 50 μs
Fj = fluorescence intensity at j-step (at 2 ms)
Fi = fluorescence intensity at i-step (at 60 ms)
Fm = maximal fluorescence intensity
Fv = Fm - Fo (maximal variable fluorescence)
Vj = (Fj - Fo) / (Fm - Fo)
Fm / Fo = Fm / Fo
Fv / Fo = Fv / Fo
Fv / Fm = Fv / Fm
Mo = TRo / RC - ETo / RC
Area = area between fluorescence curve and Fm
Sm = area / Fm - Fo (multiple turn-over)
Ss = the smallest Sm turn-over (single turn-over)
N = Sm . Mo . (I / Vj) turn-over number QA
Phi_Po = (I - Fo) / Fm (or Fv / Fm)
Phi_o = I - Vj
Phi_Eo = (I - Fo / Fm) . Phi_o
Phi_Do = 1 - Phi_Po - (Fo / Fm)
Phi_Pav = Phi_Po - (Sm / tFM); tFM = time to reach Fm (in ms)
ABS / RC = Mo . (I / Vj) . (I / Phi_Po)
TRo / RC = Mo . (I / Vj)
ETo / RC = Mo . (I / Vj) . Phi_o)
DIo / RC = (ABS / RC) - (TRo / RC)