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产品英文名称 | Human Haptoglobin Protein, His tag |
产品中文名称 | 重组人触珠蛋白,His-标签(Haptoglobin ) |
别名 | Haptoglobin |
表达宿主 | 大肠杆菌 |
种属 | 人 |
序列 | 氨基酸序列来源于: 人触珠蛋白(AAA88080.1) (Ile162-Asn406)在n端带有6×His标签。 |
蛋白长度 | 重组人触珠蛋白由245个氨基酸组成,预测分子量为27.2 kDa。 |
纯度 | > 95 % ,使用SDS-PAGE检测 |
特点&优势 | 通过LAL法测定,每微克蛋白里内毒素含量< 0.1=""> |
产品形式 | 冻干粉,冻干缓冲液为无菌的PBS, pH 7.4. |
基因ID | 3240 |
蛋白序列链接 | /uniprotkb/P00738 |
使用中注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。 |
保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。复溶后,在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液中进一步稀释(如 0.1%BSA、10%FBS、5%HSA 或 5%trehalose)。BSA、10%FBS、5%HSA 或 5% 海藻糖溶液)中进一步稀释,蛋白等分应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。 |
运输条件 | 冰袋运输(蓝冰) |
警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不建议使用我们的产品去违反任何或许可证。对于使用本产品可能发生的侵权或其他违规行为,我们不承担任何责任。 |
背景 | Haptoglobin is a glycoprotein, which is synthesized in the liver and circulates in the blood. Haptoglobin is usually produced by liver cells, but it is also produced by other tissues, such as skin, lung and kidney. It is a positive acute phase protein, which binds to free hemoglobin and removes it from circulation to prevent kidney injury and iron loss after hemolysis. Decreased levels can be seen in hemolysis and impaired liver function. A high level is a sign of acute or chronic inflammation. Hemoglobin deficiency or hypohaptoglobinemia is caused by mutation of haptoglobin gene and/or its regulatory region. Haptoglobin is also related to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, susceptibility to primary sclerosing cholangitis, idiopathic Parkinson's disease and the reduction of the incidence of plasmodium falciparum malaria. |
Fig.SDS-PAGE analysis of Human Haptoglobin Protein, His tag